Analysis Note

Positive ControlPC12, Jurkat, or HeLa cells

Application

Immunoblotting (1:1500)r>Immunofluorescence (1:500)r>Paraffin Sections (see application references)

General description

Recognizes the MYH protein and several of its approximately 10 isoforms in PC12, Jurkat, and HeLa cells.

This Anti-MYH (33-51) Rabbit pAb is validated for use in Immunoblotting, Immunofluorescence, Paraffin Sections for the detection of MYH (33-51).

Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~52-57 kDa isoforms of MYH.

Immunogen

Human

a synthetic peptide (QEGRQKHAKNNSQAKPSAC) corresponding to amino acids 33-51 of human MYH

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Wang, G., et al. 2000. Nucleic Acids Res.28, 2135.

Detects MYH and several of its approximately ten isoforms. In Jurkat cells the ~52 kDa and ~53 kDa variants were found in the nuclear fraction while the ~57 kDa protein is found in the mitochondria. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µL in Glass bottle

Physical form

Undiluted serum.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)